Imunofluorescent analysis of SSEA5 (green) and SSEA4 (red) in human episomal iPSC cells. Cells were grown on vitronectin coated plates, fixed with 4% paraformaldehyde for 15 minutes, and blocked with 3% Blocker BSA (Product # 37525) for 30 minutes at room temperature. Cells were co-stained with DyLight 488-conjugated SSEA5 and DyLight 650-conjugated SSEA4 monoclonal antibodies (top left panel, Product # MA1-144-D488 and Product # MA1-021-D650) or DyLight 488-conjugated Mouse IgG1 and DyLight 650-conjugated Mouse IgG3 isotype control antibodies (right panels, Product # MA1-191-D488 and Product # MA1-190-D650), at a dilution of 1:50 and incubated for 1 hour at room temperature. Nuclei (blue) were stained with Hoechst 33342 dye (Product # 62249). Images were taken on an EVOS FL Imaging system at 20X magnification.